The ALPINeDNA project focuses on the monitoring and conservation of biodiversity in alpine lake ecosystems in Greece, particularly in the mountainous regions of Northern and Central Pindus. These high-altitude lakes, often isolated and fragile, host some unique and sensitive species, including amphibians and aquatic invertebrates, which are particularly vulnerable to climate change and anthropogenic pressures. The primary objectives include mapping biodiversity, assessing habitat quality, and detecting potential threats, such as invasive species and hydrological changes. Through systematic field surveys, environmental DNA sampling, and collaboration with local stakeholders, the project aims to establish a robust monitoring framework. Ultimately, the goal is to support long-term conservation actions and inform environmental policy by enhancing our understanding of these ecologically important but understudied habitats.Â